MultiSciences

70-EK1149

Product Name

Human Endoglin/CD105 ELISA Kit

Customs Name

Human Endoglin/CD105 ELISA Kit

Application

ELISA

Reactivity

Human

Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates

Format

96-well strip plate

Storage

4 (unopened) standard stored at -20, others stored at 4 (opened)

Shipping Condition

4

Sample Volume

100 l

Sensitivity

3.49 pg/ml

Standard Curve Range

62.50 - 4000 pg/ml

Spike Recovery Range

95 % - 119 %

Mean Spike Recovery

1.08

CV of Intra plate

2.7 % - 4.4 %

CV of Inter plate

3.0 % - 3.5 %

Components

96-well polystyrene microplate coated with a monoclonal antibody against Endoglin

Human Endoglin Standard, lyophilized

Endoglin Detect Antibody

Streptavidin-HRP

Assay Buffer (10)

Substrate (TMB)

Stop Solution

Washing Buffer (20)

Plate Covers

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Endoglin. The Human Endoglin/CD105 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Endoglin also known as CD105 is a type I membrane glycoprotein located on cell surfaces and is part of the TGF- receptor complex. It is involved in modulating a response to the binding of TGF-1, TGF-3, activin-A, BMP-2 and BMP-7. It has been postulated that Endoglin is involved in the cytoskeletal organization affecting cell morphology and migration. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Experimental mice without the Endoglin gene die due to cardiovascular abnormalities.

In humans, Endoglin may be involved in the autosomal dominant disorder known as hereditary hemorrhagic telangiectasia (HHT) type 1. This condition leads to frequent nose bleeds, telangiectases on skin and mucosa and may cause arteriovenous malformations in different organs including brain, lung, and liver.